Beam seeks an experienced scientist to help us advance our New Opportunities programs, including discovering and validating the technical feasibility of novel genomic edits to disease-causing mutations with the ultimate goal of creating new therapeutic programs. The ideal candidate will be a self-motivated, technically competent scientist that is energized to join a small, fast-moving team.
- Critically evaluate and apply new DNA base editor, RNA editor, or nuclease constructs to prospective disease targets, with an emphasis on neurological disease.
- Generate model cell lines for novel genetic targets and optimize on-target editing efficiencies.
- Perform proof-of-concept editing in iPSC-derived, therapeutically-relevant cell types, including glutamatergic and motor neurons.
- Design and manage the execution of in vivo animal model studies for pharmacology and biodistribution.
- Design and implement cloning strategies to construct plasmids for the production of mRNA, protein, and recombinant AAV reagents used in editing experiments.
- Design and execute DNA/RNA purification and quantification assays (NGS, qPCR, ddPCR and RNA-seq).
- PhD in biochemistry, molecular biology or related discipline.
- Experience culturing and transfecting common cancer cell lines such as HEK293T and U2OS. Experience culturing iPS cells.
- Experience with molecular cloning techniques such as Gibson and USER assembly preferred.
- Experience using emerging technologies such as high-throughput sequencing and droplet digital PCR.
- Ability to design experiments, analyze data and interpret results independently and within a small, dynamic group of peers.
- Critical and creative thinking.