Beam seeks an experienced scientist to help us advance our New Opportunities programs, including discovering and validating the technical feasibility of novel genomic edits to disease-causing mutations with the ultimate goal of creating new therapeutic programs. The ideal candidate will be a self-motivated, technically competent scientist that is energized to join a small, fast-moving team.
- Critically evaluate and apply new base editor constructs to prospective disease targets.
- Generate model cell lines for novel genetic targets and optimize on-target editing efficiencies.
- Work with teams in specific therapeutic areas to translate editing to therapeutically-relevant cell types.
- Design and implement cloning strategies to construct plasmids for the production of mRNA, protein, and recombinant
- AAV reagents used in editing experiments.
- Implement novel and established assays to assess large-scale genomic rearrangements and off-target effects of base editing technologies.
- Design and execute DNA/RNA purification and quantification assays (qPCR, ddPCR and RNA-seq).
- PhD in biochemistry, molecular biology or related discipline.
- Previous lentiviral production experience.
- Experience culturing and transfecting common cancer cell lines such as HEK293T and U2OS. Experience culturing iPS cells is a plus.
- Experience with molecular cloning techniques such as Gibson and USER assembly strongly preferred.
- Experience using emerging technologies such as high-throughput sequencing and droplet digital PCR.
- Ability to design experiments, analyze data and interpret results independently and within a small, dynamic group of peers.
- Critical and creative thinking.