Beam seeks an experienced scientist to help us advance our New Opportunities programs, including precision therapeutic genome editing of patient-derived iPS and differentiated cells. The ideal candidate will support our in vitro efforts developing next-generation base editing technologies and will be a self-motivated, technically competent research associate that is energized to join a small, fast-moving team.
- Design and implement cloning strategies to construct plasmids used in gene editing experiments.
- Transfection and downstream processing of model cell lines to assess editing at disease-relevant target loci.
- Implement novel and established assays to assess large-scale genomic rearrangements and off-target effects of base editing technologies.
- Maintain patient-derived iPS cell lines.
- Employ established protocols to differentiate iPS cells into therapeutically-relevant cell types.
- Generate novel genetic edits and phenotypically characterize the resulting cell populations.
- BS/MS in cell biology, molecular biology or related discipline with 2+ years’ experience in an academic or industry lab setting.
- Experience culturing iPS cells is required.
- Molecular cloning experience, including Gibson assembly and USER cloning, is preferred.
- Ability to design experiments, analyze data and interpret results independently and within a small, dynamic group of peers.
- Critical and creative thinking.
- Experience using emerging technologies such as high-throughput sequencing and droplet digital PCR is a plus.